Modifying Enzymes
New England Biolabs, Inc. T4 RNA Ligase 2, truncated KQ – 2000 units
T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5' end of DNA or RNA to the 3' OH end of RNA. The enzyme does not use ATP for ligation but requires pre-adenylated linkers. T4 Rnl2tr R55K, K227Q is a double-point mutant of T4 RNA Ligase 2, truncated . Mutation of K227 in T4 RNA Ligase 2 reduces enzyme lysyl adenylation. K227Q reduces the formation of undesired ligation products (concatemers and circles) by T4 Rnl2tr, by reducing the trace activity of T4 Rnl2tr in transfer of adenylyl groups from linkers to the 5-phosphates of input RNAs. Mutation of R55K in T4 Rnl2tr K227Q increases the ligation activity of the enzyme to levels similar to T4 Rnl2tr. The exclusion of ATP, use of pre-adenylated linkers, and the reduced enzyme lysyl adenylation activity provide the lowest possible background in ligation reactions. This enzyme has been used for optimized linker ligation for high-throughput sequencing library construction of small RNA .
Cayman Chemical Coenzyme A sodium salt hydra
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
Cayman Chemical Coenzyme AsodIM salt hydra 5mg
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
Medchemexpress LLC Lipase, Pseudomonas fluorescens (immobilized) | 9001-62-1 | MFCD00131509 | 500mg
Lipase Pseudomonas fluorescens (Immobilized) comes from Pseudomonas fluorescens and shows good applicability in ester synthesis of food industry the biofuel synthesis the synthesis of enantiomeric pure chiral drugs and so on[1]
Worthington Biochemical Corporation BOVINE TRYPSIN DIALYZED AGAIN
Trypsin, 3X Crystallized, Dialyzed Against 1mM HCl and Lyophilized, Store at 2 deg. to 8 deg.C, Source: Bovine Pancreas, Minimum Activity: 180 units/mg Protein, 100mg
Sigma Aldrich Fine Chemicals Biosciences Topoisomerase II alpha hum
Topoisomerase II is encoded by TOP2A gene which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer ovarian cancer and prostate cancer.
Sigma Aldrich Fine Chemicals Biosciences Coenzyme Q10 d9 dimethoxy 1MG
Coenzyme Q10 d9 dimethoxy 1MG
Gold Biotechnology Inc IPTG 500 g
IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
Roche Diagnostics General Chemistry Gamma Glutamyl Transferase (GGT) For Cobas Integra Analyzer
General Chemistry Gamma Glutamyl Transferase (GGT) For Cobas Integra Analyzer.
Biotium Topoisomerase I, MT(TOP1MT/488), CF740 conjugate, 0.1mg/mL
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can
Enzo Life Sciences USP2 (catalytic domain) (rat), (recombinant) (untagged) (100 µg)
Alternative name: Ubiquitin specific protease 2. MW: ~40kDa. Purity: ≥95% (SDS-PAGE, following ion exchange chromatography). Formulation: Liquid. In 10mM MES, 0.5mM DTT, 50mM TRIS-HCl, pH 7.5, containing 20% glycerol. Source: Produced in E. coli BL21 (λDE3). Untagged USP2 catalytic domain. UniProt: Q5U349. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Long Term Storage: -80°C